Hotspot

rpl23-trnH-GUG

Genome · 174666-174946 · 281 bp

Back to Gaultheria

Pi 0.0179
MarkerSeek score 62.20
Primer available yes
Label rpl23-trnH-GUG

Feature Metadata

label_name
rpl23-trnH-GUG
feature_type
igs
region
Genome
length_bp
281
variable_sites
5
pi
0.0179
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
62.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl23-trnH-GUG_p1 CGACCCAATGCTTTATTTCTGT ATCTTAAGGGGGCGGATGTA 40.9 50.0 57.3 57.9 484 bp 1.0 58.5
rpl23-trnH-GUG_p2 CGACCCAATGCTTTATTTCTGT GATTGTGAATTCACCACGCG 40.9 50.0 57.3 58.1 443 bp 1.0 58.5
rpl23-trnH-GUG_p3 CGACCCAATGCTTTATTTCTGT TTGAAATCTTAAGGGGGCGG 40.9 50.0 57.3 57.9 489 bp 1.0 58.3
rpl23-trnH-GUG_p4 CGACCCAATGCTTTATTTCTGT GCCAAGTGGATTAAGGCAGT 40.9 50.0 57.3 58.2 464 bp 1.0 58.1
rpl23-trnH-GUG_p5 CGACCCAATGCTTTATTTCTGT GGATTGTGAATTCACCACGC 40.9 50.0 57.3 57.7 444 bp 1.0 57.4