Hotspot

trnH-GUG-rpl23

Genome · 108998-109278 · 281 bp

Back to Gaultheria

Pi 0.0179
MarkerSeek score 62.20
Primer available yes
Label trnH-GUG-rpl23

Feature Metadata

label_name
trnH-GUG-rpl23
feature_type
igs
region
Genome
length_bp
281
variable_sites
5
pi
0.0179
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
62.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnH-GUG-rpl23_p1 ATCTTAAGGGGGCGGATGTA CGACCCAATGCTTTATTTCTGT 50.0 40.9 57.9 57.3 484 bp 1.0 58.5
trnH-GUG-rpl23_p2 GATTGTGAATTCACCACGCG CGACCCAATGCTTTATTTCTGT 50.0 40.9 58.1 57.3 443 bp 1.0 58.5
trnH-GUG-rpl23_p3 TTGAAATCTTAAGGGGGCGG CGACCCAATGCTTTATTTCTGT 50.0 40.9 57.9 57.3 489 bp 1.0 58.3
trnH-GUG-rpl23_p4 GCCAAGTGGATTAAGGCAGT CGACCCAATGCTTTATTTCTGT 50.0 40.9 58.2 57.3 464 bp 1.0 58.1
trnH-GUG-rpl23_p5 GGATTGTGAATTCACCACGC CGACCCAATGCTTTATTTCTGT 50.0 40.9 57.7 57.3 444 bp 1.0 57.4