Hotspot

trnE-UUC-petA

Genome · 82947-84422 · 1476 bp

Back to Gaultheria

Pi 0.0102

MarkerSeek score 64.70

Primer available yes

Label trnE-UUC-petA

Feature Metadata

label_name: trnE-UUC-petA
feature_type: igs
region: Genome
length_bp: 1476
variable_sites: 15
pi: 0.0102
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-petA_p1	GCTGCCTCCTTGAAAGAGAG	TAGCATCTGTTGTTGTGGCA	55.0	45.0	58.3	57.7	1610-1637 bp	1.0	76.5
trnE-UUC-petA_p2	TGTCCTGAACCACTAGACGA	TAGCATCTGTTGTTGTGGCA	50.0	45.0	57.7	57.7	1589-1616 bp	1.0	76.4
trnE-UUC-petA_p3	GCTGCCTCCTTGAAAGAGAG	TCCATTACGTCTTCAAGGCC	55.0	50.0	58.3	57.6	1642-1669 bp	1.0	75.5
trnE-UUC-petA_p4	GCTGCCTCCTTGAAAGAGAG	CCATTACGTCTTCAAGGCCT	55.0	50.0	58.3	57.6	1641-1668 bp	1.0	75.4
trnE-UUC-petA_p5	TGTCCTGAACCACTAGACGA	TCCATTACGTCTTCAAGGCC	50.0	50.0	57.7	57.6	1621-1648 bp	1.0	75.4