Hotspot

psbB-psbJ

Genome · 67667-71088 · 3422 bp

Back to Gaultheria

Pi 0.00775

MarkerSeek score 61.30

Primer available yes

Label psbB-psbJ

Feature Metadata

label_name: psbB-psbJ
feature_type: igs
region: Genome
length_bp: 3422
variable_sites: 26
pi: 0.00775
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 61.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbB-psbJ_p1	GGCGGTTGTGGATGAAAATG	AAGCGCCAGACAAAGGATAG	50.0	50.0	58.0	58.0	984-1023 bp	1.0	84.6
psbB-psbJ_p2	GGCGGTTGTGGATGAAAATG	TGTTCCAACCACCGTGTAAG	50.0	50.0	58.0	58.0	1001-1040 bp	1.0	84.5
psbB-psbJ_p3	AGGCGGTTGTGGATGAAAAT	AAGCGCCAGACAAAGGATAG	45.0	50.0	58.1	58.0	985-1024 bp	1.0	84.0
psbB-psbJ_p4	AGGCGGTTGTGGATGAAAAT	TGTTCCAACCACCGTGTAAG	45.0	50.0	58.1	58.0	1002-1041 bp	1.0	83.9
psbB-psbJ_p5	CTAAGGCGGTTGTGGATGAA	AAGCGCCAGACAAAGGATAG	50.0	50.0	57.9	58.0	988-1027 bp	1.0	83.8