Hotspot

trnT-GGU-petD

Genome · 59494-62412 · 2919 bp

Back to Gaultheria

Pi 0.00994

MarkerSeek score 52.20

Primer available yes

Label trnT-GGU-petD

Feature Metadata

label_name: trnT-GGU-petD
feature_type: igs
region: Genome
length_bp: 2919
variable_sites: 29
pi: 0.00994
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-petD_p1	TGGCGTTACTCTACCACTGA	TGGCGCAACATTACCAATTG	50.0	45.0	58.1	57.6	2999 bp	0.5	56.4
trnT-GGU-petD_p2	TGGCGTTACTCTACCACTGA	GGCGCAACATTACCAATTGA	50.0	45.0	58.1	57.4	2998 bp	0.5	54.1
trnT-GGU-petD_p3	GGCGTTACTCTACCACTGAG	TGGCGCAACATTACCAATTG	55.0	45.0	57.4	57.6	2998 bp	0.5	52.6
trnT-GGU-petD_p4	TGGCGTTACTCTACCACTGA	TTGGCGCAACATTACCAATT	50.0	40.0	58.1	56.9	3000 bp	0.5	50.4
trnT-GGU-petD_p5	GGCGTTACTCTACCACTGAG	GGCGCAACATTACCAATTGA	55.0	45.0	57.4	57.4	2997 bp	0.5	50.3