Hotspot

trnV-GAC-rps12

Genome · 128-1592 · 1465 bp

Back to Gaultheria

Pi 0.011

MarkerSeek score 54.10

Primer available yes

Label trnV-GAC-rps12

Feature Metadata

label_name: trnV-GAC-rps12
feature_type: igs
region: Genome
length_bp: 1465
variable_sites: 16
pi: 0.011
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnV-GAC-rps12_p1	GATGACTTCCACCACGTCAA	TGGCAACTTTACGTAAGGCA	50.0	45.0	57.8	57.7	1561-1596 bp	1.0	77.7
trnV-GAC-rps12_p2	AACTGATGACTTCCACCACG	TGGCAACTTTACGTAAGGCA	50.0	45.0	57.8	57.7	1565-1600 bp	1.0	77.6
trnV-GAC-rps12_p3	ATGACTTCCACCACGTCAAG	TGGCAACTTTACGTAAGGCA	50.0	45.0	57.8	57.7	1560-1595 bp	1.0	77.6
trnV-GAC-rps12_p4	CTGATGACTTCCACCACGTC	TGGCAACTTTACGTAAGGCA	55.0	45.0	58.3	57.7	1563-1598 bp	1.0	76.7
trnV-GAC-rps12_p5	GGCTCGAACTGATGACTTCC	TGGCAACTTTACGTAAGGCA	55.0	45.0	58.4	57.7	1571-1606 bp	1.0	76.2