Hotspot

ycf1

SSC · 38847-43562 · 4716 bp

Back to Gastrolobium

Pi 0.0546

MarkerSeek score 68.00

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 4716
variable_sites: 257
pi: 0.0546
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	CAGACGCACTGGTTCGATAA	GGAGCACGGGGAATATTTCA	50.0	50.0	58.0	57.7	1179 bp	0.5	57.2
ycf1_p2	CAGACGCACTGGTTCGATAA	TGGAGCACGGGGAATATTTC	50.0	50.0	58.0	57.7	1180 bp	0.5	57.2
ycf1_p3	CCCGTTCGACGTCACATAAT	GGAGCACGGGGAATATTTCA	50.0	50.0	58.1	57.7	858 bp	0.5	56.6
ycf1_p4	CCCGTTCGACGTCACATAAT	TGGAGCACGGGGAATATTTC	50.0	50.0	58.1	57.7	859 bp	0.5	56.6
ycf1_p5	TCTTGGTTTGAGAGGGGTGA	GGAGCACGGGGAATATTTCA	50.0	50.0	58.2	57.7	821 bp	0.5	55.8