Hotspot

ycf1-trnN-GUU

SSC · 43563-44559 · 997 bp

Back to Gastrolobium

Pi 0.0373

MarkerSeek score 68.90

Primer available yes

Label ycf1-trnN-GUU

Feature Metadata

label_name: ycf1-trnN-GUU
feature_type: igs
region: SSC
length_bp: 997
variable_sites: 36
pi: 0.0373
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 68.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1-trnN-GUU_p1	TCCAGAACTTTTGTTGCCCT	ACACCGAAGTATTGACGACG	45.0	50.0	57.5	58.0	1796 bp	0.5	56.2
ycf1-trnN-GUU_p2	TCCAGAACTTTTGTTGCCCT	TGCCCTACTCTTGGAATCCA	45.0	50.0	57.5	58.0	2080 bp	0.5	56.1
ycf1-trnN-GUU_p3	TCCAGAACTTTTGTTGCCCT	GGCAGAATAGCAGAGCAAGT	45.0	50.0	57.5	58.0	2013 bp	0.5	56.1
ycf1-trnN-GUU_p4	TCCAGAACTTTTGTTGCCCT	GGTAATTGTGGCCTCTCAGG	45.0	55.0	57.5	58.0	1928 bp	0.5	56.0
ycf1-trnN-GUU_p5	TCCAGAACTTTTGTTGCCCT	AATGCGTTCCTCGTCATGAA	45.0	45.0	57.5	57.9	1966 bp	0.5	55.6