Hotspot

rps15-ycf1

SSC · 38509-38846 · 338 bp

Back to Gastrolobium

Pi 0.059

MarkerSeek score 71.00

Primer available yes

Label rps15-ycf1

Feature Metadata

label_name: rps15-ycf1
feature_type: igs
region: SSC
length_bp: 338
variable_sites: 18
pi: 0.059
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.90
markerseek_score: 71.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ycf1_p1	AACACCATGCATTGATGGGT	TTATCGAACCAGTGCGTCTG	45.0	50.0	58.1	58.0	1369-1396 bp	1.0	85.5
rps15-ycf1_p2	TGCATTGATGGGTGATGAGG	TTATCGAACCAGTGCGTCTG	50.0	50.0	57.9	58.0	1362-1389 bp	1.0	85.5
rps15-ycf1_p3	TCCCGGGTCTTCTTCTCTTT	TTATCGAACCAGTGCGTCTG	50.0	50.0	58.0	58.0	994 bp	0.5	59.7
rps15-ycf1_p4	TCCCGGGTCTTCTTCTCTTT	GAACCAGTGCGTCTGTCTAG	50.0	55.0	58.0	58.0	989 bp	0.5	59.7
rps15-ycf1_p5	GCATTGATGGGTGATGAGGT	TTATCGAACCAGTGCGTCTG	50.0	50.0	57.9	58.0	1388 bp	0.5	59.4