Hotspot

trnL-UAG-ccsA

SSC · 28975-29092 · 118 bp

Back to Gastrolobium

Pi 0.0521

MarkerSeek score 73.20

Primer available yes

Label trnL-UAG-ccsA

Feature Metadata

label_name: trnL-UAG-ccsA
feature_type: igs
region: SSC
length_bp: 118
variable_sites: 5
pi: 0.0521
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.81
markerseek_score: 73.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAG-ccsA_p1	TAGACACGCTGCTCTTAGGA	CGATCGAAAACGATAGATGGG	50.0	47.6	57.9	56.7	209-231 bp	1.0	66.3
trnL-UAG-ccsA_p2	TAGACACGCTGCTCTTAGGA	ACGATCGAAAACGATAGATGGG	50.0	45.5	57.9	58.4	210-232 bp	1.0	65.9
trnL-UAG-ccsA_p3	TAGACACGCTGCTCTTAGGA	CGATCGAAAACGATAGATGGGT	50.0	45.5	57.9	58.4	209-231 bp	1.0	65.9
trnL-UAG-ccsA_p4	AAATTGGTAGACACGCTGCT	CGATCGAAAACGATAGATGGG	45.0	47.6	57.8	56.7	216-238 bp	1.0	65.8
trnL-UAG-ccsA_p5	GTAGACACGCTGCTCTTAGG	CGATCGAAAACGATAGATGGG	55.0	47.6	57.8	56.7	210-232 bp	1.0	65.7