Hotspot

rpl16

LSC · 82900-84558 · 1659 bp

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Pi 0.0138

MarkerSeek score 62.80

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1659
variable_sites: 81
pi: 0.0138
unique_haplotype_count: 17
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 62.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	TGGGCACTGCTTCTTTGATT	GCAAATTGCAGGACGTATCG	45.0	50.0	58.0	57.9	2205-2333 bp	1.0	86.3
rpl16_p2	TTGGGCACTGCTTCTTTGAT	GCAAATTGCAGGACGTATCG	45.0	50.0	58.0	57.9	2206-2334 bp	1.0	86.3
rpl16_p3	ATTGGGCACTGCTTCTTTGA	GAACACGGGGAATCGAAGAA	45.0	50.0	58.0	57.9	2430-2558 bp	1.0	85.9
rpl16_p4	TGGGCACTGCTTCTTTGATT	GAACACGGGGAATCGAAGAA	45.0	50.0	58.0	57.9	2428-2556 bp	1.0	85.9
rpl16_p5	TTGGGCACTGCTTCTTTGAT	GAACACGGGGAATCGAAGAA	45.0	50.0	58.0	57.9	2429-2557 bp	1.0	85.9