Hotspot

psaC-rps15

SSC · 115468-116826 · 1359 bp

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Pi 0.0159

MarkerSeek score 62.60

Primer available yes

Label psaC-rps15

Feature Metadata

label_name: psaC-rps15
feature_type: igs
region: SSC
length_bp: 1359
variable_sites: 88
pi: 0.0159
unique_haplotype_count: 16
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC-rps15_p1	GGACAAGCGGATTCACATCT	ATCACAAAGGGGTCTACGAA	50.0	45.0	58.0	56.2	1251-1717 bp	1.0	73.7
psaC-rps15_p2	GGACAAGCGGATTCACATCT	TCACAAAGGGGTCTACGAAT	50.0	45.0	58.0	56.2	1250-1716 bp	1.0	73.7
psaC-rps15_p3	AGCTCGAACACATTGGGTAC	ATCACAAAGGGGTCTACGAA	50.0	45.0	57.9	56.2	1135-1601 bp	1.0	73.5
psaC-rps15_p4	AGCTCGAACACATTGGGTAC	TCACAAAGGGGTCTACGAAT	50.0	45.0	57.9	56.2	1134-1600 bp	1.0	73.5
psaC-rps15_p5	GCTCGAACACATTGGGTACA	TCACAAAGGGGTCTACGAAT	50.0	45.0	58.2	56.2	1133-1599 bp	1.0	72.7