Hotspot

rpl32-trnL-UAG

SSC · 111899-112486 · 588 bp

Back to Gastrochilus

Pi 0.0266
MarkerSeek score 69.50
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
588
variable_sites
55
pi
0.0266
unique_haplotype_count
16
primer_available
yes
alignment_reliability
1.00
markerseek_score
69.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 TCCACCGGACAGTCAAAAAG TGGCGAAACCTTGGGTAAAA 50.0 45.0 58.0 57.9 1169-1231 bp 1.0 87.9
rpl32-trnL-UAG_p2 GAGTCCGAAGCTTCTTTCGT ATCCGCATAAAAGAGCTGCA 50.0 45.0 57.9 58.0 1903-2128 bp 1.0 87.8
rpl32-trnL-UAG_p3 GAGTCCGAAGCTTCTTTCGT TCCGCATAAAAGAGCTGCAT 50.0 45.0 57.9 58.0 1902-2127 bp 1.0 87.8
rpl32-trnL-UAG_p4 TCCACCGGACAGTCAAAAAG TCCGCATAAAAGAGCTGCAT 50.0 45.0 58.0 58.0 1288-1350 bp 1.0 87.7
rpl32-trnL-UAG_p5 TCCACCGGACAGTCAAAAAG ATCCGCATAAAAGAGCTGCA 50.0 45.0 58.0 58.0 1289-1351 bp 1.0 87.6