Hotspot

trnN-GUU-rpl32

IRb · 111216-111724 · 509 bp

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Pi 0.0233

MarkerSeek score 66.30

Primer available yes

Label trnN-GUU-rpl32

Feature Metadata

label_name: trnN-GUU-rpl32
feature_type: igs
region: IRb
length_bp: 509
variable_sites: 51
pi: 0.0233
unique_haplotype_count: 14
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnN-GUU-rpl32_p1	TCAAAGCGAGCCCTTTCATT	CTTTTTGACTGTCCGGTGGA	45.0	50.0	58.1	58.0	763-979 bp	1.0	86.6
trnN-GUU-rpl32_p2	AAAGCGAGCCCTTTCATTCA	CTTTTTGACTGTCCGGTGGA	45.0	50.0	58.1	58.0	761-977 bp	1.0	86.5
trnN-GUU-rpl32_p3	AAGCGAGCCCTTTCATTCAA	CTTTTTGACTGTCCGGTGGA	45.0	50.0	58.1	58.0	760-976 bp	1.0	86.5
trnN-GUU-rpl32_p4	TATGCCGAGGTATTGAGGGT	CTTTTTGACTGTCCGGTGGA	50.0	50.0	57.9	58.0	873-1089 bp	1.0	85.8
trnN-GUU-rpl32_p5	GGCAGAATAGCAGAGCAAGT	CTTTTTGACTGTCCGGTGGA	50.0	50.0	58.0	58.0	1037-1253 bp	1.0	85.5