Hotspot

psbE-petL

LSC · 65450-66598 · 1149 bp

Back to Gastrochilus

Pi 0.0118

MarkerSeek score 62.00

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1149
variable_sites: 54
pi: 0.0118
unique_haplotype_count: 16
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	CAACCCGCAATGAATAGGGA	GCTGGGGTTATAGTTGAAGCA	50.0	47.6	57.9	58.0	1299-1330 bp	1.0	76.9
psbE-petL_p2	CAACCCGCAATGAATAGGGA	GGGTTATAGTTGAAGCAGCCA	50.0	47.6	57.9	58.0	1295-1326 bp	1.0	76.9
psbE-petL_p3	CAACCCGCAATGAATAGGGA	TGGGGTTATAGTTGAAGCAGC	50.0	47.6	57.9	58.0	1297-1328 bp	1.0	76.9
psbE-petL_p4	ACTGACGAATAACCAACCCG	GGGTTATAGTTGAAGCAGCCA	50.0	47.6	57.9	58.0	1308-1339 bp	1.0	76.6
psbE-petL_p5	ACTGACGAATAACCAACCCG	TGGGGTTATAGTTGAAGCAGC	50.0	47.6	57.9	58.0	1310-1341 bp	1.0	76.6