Hotspot

petA-psbJ

LSC · 63744-64693 · 950 bp

Back to Gastrochilus

Pi 0.0175
MarkerSeek score 67.60
Primer available yes
Label petA-psbJ

Feature Metadata

label_name
petA-psbJ
feature_type
igs
region
LSC
length_bp
950
variable_sites
59
pi
0.0175
unique_haplotype_count
16
primer_available
yes
alignment_reliability
1.00
markerseek_score
67.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petA-psbJ_p1 TGAAAAGGTTCAGTTGTACGA TATTCCGGGTTGGGTTCATC 38.1 50.0 55.4 57.3 970-1015 bp 1.0 54.3
petA-psbJ_p2 TGAAAAGGTTCAGTTGTACGA CTATTCCGGGTTGGGTTCAT 38.1 50.0 55.4 57.3 971-1016 bp 1.0 54.2
petA-psbJ_p3 ACAGTTTGAAAAGGTTCAGTTGT TATTCCGGGTTGGGTTCATC 34.8 50.0 57.3 57.3 976-1021 bp 1.0 53.2
petA-psbJ_p4 ACAGTTTGAAAAGGTTCAGTTGT CTATTCCGGGTTGGGTTCAT 34.8 50.0 57.3 57.3 977-1022 bp 1.0 53.1
petA-psbJ_p5 AGGTTCAGTTGTACGAAATGA TATTCCGGGTTGGGTTCATC 38.1 50.0 55.2 57.3 965-1010 bp 1.0 52.5