Hotspot

ndhC-trnV-UAC

LSC · 51192-51853 · 662 bp

Back to Gastrochilus

Pi 0.0176

MarkerSeek score 63.30

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 662
variable_sites: 48
pi: 0.0176
unique_haplotype_count: 15
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 63.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	AGGAATGCCCAGAAAATATCA	AGAAGGTCTACGGTTCGAGT	38.1	50.0	54.9	57.8	720-783 bp	0.823529	46.3
ndhC-trnV-UAC_p2	AGGAATGCCCAGAAAATATCA	GAAGGTCTACGGTTCGAGTC	38.1	55.0	54.9	57.5	719-782 bp	0.823529	43.5
ndhC-trnV-UAC_p3	AGGAATGCCCAGAAAATATCAT	AGAAGGTCTACGGTTCGAGT	36.4	50.0	55.5	57.8	720-783 bp	0.823529	42.9
ndhC-trnV-UAC_p4	AGGAATGCCCAGAAAATATCA	GTTCGAGTCCGTATAGCCCT	38.1	55.0	54.9	58.7	708-771 bp	0.823529	42.4
ndhC-trnV-UAC_p5	TGCCCAGAAAATATCATATTCGTG	AGAAGGTCTACGGTTCGAGT	37.5	50.0	57.4	57.8	715-778 bp	0.764706	39.8