Hotspot

rpoB-trnC-GCA

LSC · 27505-29135 · 1631 bp

Back to Gastrochilus

Pi 0.0273

MarkerSeek score 67.60

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1631
variable_sites: 139
pi: 0.0273
unique_haplotype_count: 15
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 67.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	CCAAATTAAGACCGCGGGTA	CCGGGGATAAAGGATTTGCA	50.0	50.0	58.0	57.9	1819-1985 bp	1.0	89.4
rpoB-trnC-GCA_p2	CCAAATTAAGACCGCGGGTA	AGTATCGACAGACCTAGCCC	50.0	55.0	58.0	58.0	1939-2105 bp	0.941176	87.0
rpoB-trnC-GCA_p3	CCAAATTAAGACCGCGGGTA	GTATCGACAGACCTAGCCCT	50.0	55.0	58.0	58.0	1938-2104 bp	0.941176	87.0
rpoB-trnC-GCA_p4	CCCCTACACAGGCAAATTGT	AGTATCGACAGACCTAGCCC	50.0	55.0	58.1	58.0	2378-2544 bp	0.941176	85.7
rpoB-trnC-GCA_p5	CCCCTACACAGGCAAATTGT	GTATCGACAGACCTAGCCCT	50.0	55.0	58.1	58.0	2377-2543 bp	0.941176	85.7