Hotspot

matK-trnK-UUU

LSC · 3252-4478 · 1227 bp

Back to Gastrochilus

Pi 0.0333

MarkerSeek score 74.80

Primer available yes

Label matK-trnK-UUU

Feature Metadata

label_name: matK-trnK-UUU
feature_type: igs
region: LSC
length_bp: 1227
variable_sites: 129
pi: 0.0333
unique_haplotype_count: 16
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 74.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK-trnK-UUU_p1	CCAGATGGATGGGATAGGGT	TCCGGGTTGCTAACTCAATG	55.0	50.0	57.9	57.9	1685-1784 bp	1.0	89.7
matK-trnK-UUU_p2	CCAGATGGATGGGATAGGGT	CGGGTTGCTAACTCAATGGT	55.0	50.0	57.9	58.2	1683-1782 bp	1.0	89.1
matK-trnK-UUU_p3	CCAGATGGATGGGATAGGGT	TCGATCCCGAAGAGAAGGAA	55.0	50.0	57.9	57.9	2379-2475 bp	1.0	88.5
matK-trnK-UUU_p4	CCAGATGGATGGGATAGGGT	GCTCAACCCACAGAAACTGT	55.0	50.0	57.9	58.3	2270-2366 bp	1.0	87.3
matK-trnK-UUU_p5	CCAGATGGATGGGATAGGGT	GTGCTCAACCCACAGAAACT	55.0	50.0	57.9	58.3	2272-2368 bp	1.0	87.3