Hotspot

ycf1

SSC · 128311-133500 · 5190 bp

Back to Garuga

Pi 0.000385
MarkerSeek score 40.20
Primer available yes
Label ycf1

Feature Metadata

label_name
ycf1
feature_type
gene
region
SSC
length_bp
5190
variable_sites
3
pi
0.000385
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
40.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf1_p1 ACCATAGTCCTTTTCTCGGT TCTCCTGCTTCGACTGAGAA 45.0 50.0 55.9 58.1 259 bp 1.0 62.5
ycf1_p2 ACCATAGTCCTTTTCTCGGT ATTGTCTCCTGCTTCGACTG 45.0 50.0 55.9 57.6 263 bp 1.0 60.2
ycf1_p3 ACCATAGTCCTTTTCTCGGT TTGTCTCCTGCTTCGACTGA 45.0 50.0 55.9 58.7 262 bp 1.0 57.8
ycf1_p4 ACCATAGTCCTTTTCTCGGT CTCCTGCTTCGACTGAGAAA 45.0 50.0 55.9 57.3 258 bp 1.0 57.3
ycf1_p5 ACCATAGTCCTTTTCTCGGT GTCTCCTGCTTCGACTGAGA 45.0 55.0 55.9 58.8 260 bp 1.0 56.5