Hotspot

rps16

LSC · 5164-6316 · 1153 bp

Pi 0.00116

MarkerSeek score 47.10

Primer available yes

Label rps16

Feature Metadata

label_name: rps16
feature_type: gene
region: LSC
length_bp: 1153
variable_sites: 2
pi: 0.00116
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16_p1	GGAGTCATCCACACCCTCTT	CTGTGTATCGACCGAAGCAA	55.0	50.0	58.7	58.0	1270-1271 bp	1.0	74.4
rps16_p2	GGAGTCATCCACACCCTCT	CTGTGTATCGACCGAAGCAA	57.9	50.0	58.0	58.0	1270-1271 bp	1.0	72.1
rps16_p3	GAGTCATCCACACCCTCTTT	CTGTGTATCGACCGAAGCAA	50.0	50.0	56.8	58.0	1269-1270 bp	1.0	70.8
rps16_p4	GGAGTCATCCACACCCTCTT	TGTGTATCGACCGAAGCAAT	55.0	45.0	58.7	57.3	1269-1270 bp	1.0	69.1
rps16_p5	GAGTCATCCACACCCTCTTTT	CTGTGTATCGACCGAAGCAA	47.6	50.0	57.6	58.0	1269-1270 bp	1.0	68.7