Hotspot

ndhD

SSC · 119954-121453 · 1500 bp

Back to Garuga

Pi 0.000444
MarkerSeek score 46.50
Primer available yes
Label ndhD

Feature Metadata

label_name
ndhD
feature_type
gene
region
SSC
length_bp
1500
variable_sites
1
pi
0.000444
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
46.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhD_p1 TGGACAACGAACAATTTTCA GCACGGGTTTTTCTGGTCTA 35.0 50.0 54.0 58.1 1603-1604 bp 1.0 46.8
ndhD_p2 TGGACAACGAACAATTTTCA ACCGACAAAAACCCGTACTC 35.0 50.0 54.0 58.1 1663-1664 bp 1.0 46.6
ndhD_p3 TGGACAACGAACAATTTTCA CGACAAAAACCCGTACTCGA 35.0 50.0 54.0 58.2 1661-1662 bp 1.0 45.8
ndhD_p4 TGGACAACGAACAATTTTCAA GCACGGGTTTTTCTGGTCTA 33.3 50.0 54.8 58.1 1603-1604 bp 1.0 45.7
ndhD_p5 TTGGACAACGAACAATTTTCA GCACGGGTTTTTCTGGTCTA 33.3 50.0 54.8 58.1 1604-1605 bp 1.0 45.7