Hotspot

cemA

LSC · 63628-64326 · 699 bp

Pi 0.000954

MarkerSeek score 46.90

Primer available yes

Label cemA

Feature Metadata

label_name: cemA
feature_type: gene
region: LSC
length_bp: 699
variable_sites: 1
pi: 0.000954
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
cemA_p1	TCGATTCATAGGCGCGATAC	ACGACCTTGAATGCTTTGCT	50.0	45.0	57.7	58.4	874 bp	1.0	74.9
cemA_p2	CGATTCATAGGCGCGATACT	ACGACCTTGAATGCTTTGCT	50.0	45.0	57.7	58.4	873 bp	1.0	74.9
cemA_p3	GAGACTCGATTCATAGGCGC	ACGACCTTGAATGCTTTGCT	55.0	45.0	58.3	58.4	879 bp	1.0	74.5
cemA_p4	CAACGGATGAGGTGGATTCA	ACGACCTTGAATGCTTTGCT	50.0	45.0	57.6	58.4	807 bp	1.0	73.8
cemA_p5	GTCAACGGATGAGGTGGATT	ACGACCTTGAATGCTTTGCT	50.0	45.0	57.6	58.4	809 bp	1.0	73.7