Hotspot

accD

LSC · 59761-61260 · 1500 bp

Back to Garuga

Pi 0.000444
MarkerSeek score 46.50
Primer available yes
Label accD

Feature Metadata

label_name
accD
feature_type
gene
region
LSC
length_bp
1500
variable_sites
1
pi
0.000444
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
46.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
accD_p1 TGATACTTCGGGGTCTCACA TCTGCATGATTCTTTCGACA 50.0 40.0 57.8 55.1 1662 bp 1.0 55.0
accD_p2 TGATACTTCGGGGTCTCACA TCTGCATGATTCTTTCGACAA 50.0 38.1 57.8 55.9 1662 bp 1.0 53.5
accD_p3 AGTGATACTTCGGGGTCTCA TCTGCATGATTCTTTCGACA 50.0 40.0 57.5 55.1 1664 bp 1.0 52.6
accD_p4 GTGATACTTCGGGGTCTCAC TCTGCATGATTCTTTCGACA 55.0 40.0 57.4 55.1 1663 bp 1.0 52.2
accD_p5 TGATACTTCGGGGTCTCACA ATCTGCATGATTCTTTCGACA 50.0 38.1 57.8 55.7 1663 bp 1.0 51.7