Hotspot

rpoA-rps11

LSC · 82086-82150 · 65 bp

Back to Garuga

Pi 0.0103
MarkerSeek score 46.50
Primer available yes
Label rpoA-rps11

Feature Metadata

label_name
rpoA-rps11
feature_type
igs
region
LSC
length_bp
65
variable_sites
1
pi
0.0103
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
46.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpoA-rps11_p1 ACTTCCACTGTAGTGTCCGA AGAGATGCAGCATTACGAGC 50.0 50.0 58.0 58.1 228 bp 1.0 79.2
rpoA-rps11_p2 ACTTCCACTGTAGTGTCCGA GAGATGCAGCATTACGAGCT 50.0 50.0 58.0 58.1 227 bp 1.0 79.2
rpoA-rps11_p3 TTCCACTGTAGTGTCCGAGT AGAGATGCAGCATTACGAGC 50.0 50.0 58.0 58.1 226 bp 1.0 79.2
rpoA-rps11_p4 TTCCACTGTAGTGTCCGAGT GAGATGCAGCATTACGAGCT 50.0 50.0 58.0 58.1 225 bp 1.0 79.2
rpoA-rps11_p5 CACTTCCACTGTAGTGTCCG GAGATGCAGCATTACGAGCT 55.0 50.0 57.9 58.1 228 bp 1.0 78.7