Hotspot

petA-psbJ

LSC · 65503-66600 · 1098 bp

Back to Garuga

Pi 0.00121
MarkerSeek score 47.00
Primer available yes
Label petA-psbJ

Feature Metadata

label_name
petA-psbJ
feature_type
igs
region
LSC
length_bp
1098
variable_sites
2
pi
0.00121
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
47.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petA-psbJ_p1 ATCCATTACGTGTCCAAGGC TATTCCGGATTGGGTTCGTC 50.0 50.0 58.0 57.4 1244 bp 1.0 75.0
petA-psbJ_p2 CATTACGTGTCCAAGGCCTT TATTCCGGATTGGGTTCGTC 50.0 50.0 58.2 57.4 1241 bp 1.0 73.8
petA-psbJ_p3 ATCCATTACGTGTCCAAGGC GGATTGGGTTCGTCCTTGTA 50.0 50.0 58.0 57.2 1238 bp 1.0 73.6
petA-psbJ_p4 CATTACGTGTCCAAGGCCTT GGATTGGGTTCGTCCTTGTA 50.0 50.0 58.2 57.2 1235 bp 1.0 72.5
petA-psbJ_p5 TTACGTGTCCAAGGCCTTTT TATTCCGGATTGGGTTCGTC 45.0 50.0 57.6 57.4 1239 bp 1.0 72.5