Hotspot

psaA-pafI

LSC · 43805-44505 · 701 bp

Back to Garuga

Pi 0
MarkerSeek score 46.40
Primer available yes
Label psaA-pafI

Feature Metadata

label_name
psaA-pafI
feature_type
igs
region
LSC
length_bp
701
variable_sites
0
pi
0
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
46.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psaA-pafI_p1 GCCCATTCCTCGAAAGAAGT TATCGCGCTTACTCCTGGTA 50.0 50.0 57.9 58.0 850-851 bp 1.0 78.9
psaA-pafI_p2 GCCCATTCCTCGAAAGAAGT CGCGCTTACTCCTGGTAATT 50.0 50.0 57.9 58.1 847-848 bp 1.0 78.7
psaA-pafI_p3 GCCCATTCCTCGAAAGAAGT AGCGCAGAATTGGTTGAAGA 50.0 45.0 57.9 58.1 820-821 bp 1.0 78.3
psaA-pafI_p4 GCCCATTCCTCGAAAGAAGT ATTGGTTGAAGATCACGGGG 50.0 50.0 57.9 57.9 812-813 bp 1.0 78.1
psaA-pafI_p5 TTGAGAAATGACCGGGTCTG TATCGCGCTTACTCCTGGTA 50.0 50.0 57.5 58.0 870-871 bp 1.0 76.1