Hotspot

trnT-GGU-psbD

LSC · 33057-34560 · 1504 bp

Back to Garuga

Pi 0.00266
MarkerSeek score 47.90
Primer available yes
Label trnT-GGU-psbD

Feature Metadata

label_name
trnT-GGU-psbD
feature_type
igs
region
LSC
length_bp
1504
variable_sites
6
pi
0.00266
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
47.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-GGU-psbD_p1 GTGGTAGAGTAACGCCATGG GAAACGATCCCTCCGTAACC 55.0 55.0 58.1 58.1 1646 bp 1.0 79.2
trnT-GGU-psbD_p2 TCAGTGGTAGAGTAACGCCA GAAACGATCCCTCCGTAACC 50.0 55.0 58.1 58.1 1649 bp 1.0 79.1
trnT-GGU-psbD_p3 GTGGTAGAGTAACGCCATGG ATCCCTCCGTAACCAGTCAT 55.0 50.0 58.1 57.8 1640 bp 1.0 78.4
trnT-GGU-psbD_p4 TCAGTGGTAGAGTAACGCCA ATCCCTCCGTAACCAGTCAT 50.0 50.0 58.1 57.8 1643 bp 1.0 78.2
trnT-GGU-psbD_p5 TCCTATCTGCCCAGTGACTC GAAACGATCCCTCCGTAACC 55.0 55.0 58.2 58.1 1701 bp 1.0 78.0