Hotspot

petN-psbM

LSC · 30064-30789 · 726 bp

Back to Garuga

Pi 0.000918
MarkerSeek score 46.80
Primer available yes
Label petN-psbM

Feature Metadata

label_name
petN-psbM
feature_type
igs
region
LSC
length_bp
726
variable_sites
1
pi
0.000918
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
46.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petN-psbM_p1 TAGTTAGTCTCGCTTGGGCT TGCTACTGCACTGTTCATTCT 50.0 42.9 58.2 57.6 888 bp 1.0 67.6
petN-psbM_p2 ATGGGGAAGAAGTGGACTCT TGCTACTGCACTGTTCATTCT 50.0 42.9 57.6 57.6 827 bp 1.0 66.0
petN-psbM_p3 TAGTTAGTCTCGCTTGGGCT TGCTACTGCACTGTTCATTC 50.0 45.0 58.2 56.1 888 bp 1.0 63.4
petN-psbM_p4 TAGTTAGTCTCGCTTGGGCT ACGTAAAAACGGTTAGCCAA 50.0 40.0 58.2 55.9 838 bp 1.0 62.2
petN-psbM_p5 ATGGGGAAGAAGTGGACTCT TGCTACTGCACTGTTCATTC 50.0 45.0 57.6 56.1 827 bp 1.0 61.9