Hotspot

rpoC2

LSC · 27009-31565 · 4557 bp

Back to Garnotia

Pi 0.00523

MarkerSeek score 33.20

Primer available yes

Label rpoC2

Feature Metadata

label_name: rpoC2
feature_type: gene
region: LSC
length_bp: 4557
variable_sites: 43
pi: 0.00523
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 33.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC2_p1	GCGAAGAAATGGGTTCGTTG	CCTATACTCTTCCCCTCGGG	50.0	60.0	58.0	58.1	235-256 bp	1.0	80.7
rpoC2_p2	AAGCGAAGAAATGGGTTCGT	CCTATACTCTTCCCCTCGGG	45.0	60.0	58.1	58.1	237-258 bp	1.0	80.2
rpoC2_p3	AGCGAAGAAATGGGTTCGTT	CCTATACTCTTCCCCTCGGG	45.0	60.0	58.1	58.1	236-257 bp	1.0	80.2
rpoC2_p4	GTTGGCGAAAAAGCGAAGAA	CCTATACTCTTCCCCTCGGG	45.0	60.0	57.9	58.1	247-268 bp	1.0	80.1
rpoC2_p5	TTGTTGGCGAAAAAGCGAAG	CCTATACTCTTCCCCTCGGG	45.0	60.0	58.2	58.1	249-270 bp	1.0	79.6