Hotspot

ndhF

SSC · 104894-107110 · 2217 bp

Back to Garnotia

Pi 0.00579

MarkerSeek score 48.90

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2217
variable_sites: 23
pi: 0.00579
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	TGCATTTTGATGAGCCTCGT	AGGTTTGGTGAATCGGAAACA	45.0	42.9	58.2	58.1	2336-2342 bp	1.0	70.9
ndhF_p2	GTTTGCTGGCTTATTTGGCA	AGGTTTGGTGAATCGGAAACA	45.0	42.9	57.5	58.1	2480-2495 bp	1.0	69.2
ndhF_p3	ACGGAGACTTGCTTCACATT	AGGTTTGGTGAATCGGAAACA	45.0	42.9	57.4	58.1	2566-2581 bp	1.0	68.3
ndhF_p4	GAGCCGAAAGATGGATGCAT	AGGTTTGGTGAATCGGAAACA	50.0	42.9	58.4	58.1	2987-2996 bp	0.75	59.5
ndhF_p5	CTCGAGCCGAAAGATGGATG	AGGTTTGGTGAATCGGAAACA	55.0	42.9	58.5	58.1	2990-2999 bp	0.75	58.6