Hotspot

ndhF-rpl32

SSC · 107111-107924 · 814 bp

Back to Garnotia

Pi 0.00884

MarkerSeek score 55.60

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 814
variable_sites: 14
pi: 0.00884
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	CTGGAAGTGGGAGAAGAGGA	TCCAAAGATTTTTACGAATACGCT	55.0	33.3	58.1	57.3	927-935 bp	1.0	43.2
ndhF-rpl32_p2	CTGGAAGTGGGAGAAGAGGA	TGACATCGAAGTACGTTTTT	55.0	35.0	58.1	53.2	899-907 bp	1.0	42.9
ndhF-rpl32_p3	CTGGAAGTGGGAGAAGAGGA	TTGACATCGAAGTACGTTTT	55.0	35.0	58.1	53.2	900-908 bp	1.0	42.9
ndhF-rpl32_p4	CTGGAAGTGGGAGAAGAGGA	TTTGACATCGAAGTACGTTT	55.0	35.0	58.1	53.2	901-909 bp	1.0	42.9
ndhF-rpl32_p5	CTGGAAGTGGGAGAAGAGGA	TTTTGACATCGAAGTACGTT	55.0	35.0	58.1	53.2	902-910 bp	1.0	42.9