Hotspot

rpl33-rps18

LSC · 67232-67519 · 288 bp

Back to Garnotia

Pi 0.00926

MarkerSeek score 52.80

Primer available yes

Label rpl33-rps18

Feature Metadata

label_name: rpl33-rps18
feature_type: igs
region: LSC
length_bp: 288
variable_sites: 5
pi: 0.00926
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl33-rps18_p1	AGAATCGCCACAATACACCC	TGGATTTACGAAAGGGTTGCT	50.0	42.9	58.0	57.9	435-439 bp	1.0	71.8
rpl33-rps18_p2	CCACAATACACCCGGACAAT	TGGATTTACGAAAGGGTTGCT	50.0	42.9	57.9	57.9	428-432 bp	1.0	71.1
rpl33-rps18_p3	TGTCGTTATTGTCGCAAGCA	TGGATTTACGAAAGGGTTGCT	45.0	42.9	58.5	57.9	391-395 bp	1.0	68.3
rpl33-rps18_p4	AGAATCGCCACAATACACCC	TGGATTTACGAAAGGGTTGC	50.0	45.0	58.0	56.3	435-439 bp	1.0	67.6
rpl33-rps18_p5	TTGTCGTTATTGTCGCAAGC	TGGATTTACGAAAGGGTTGCT	45.0	42.9	57.4	57.9	392-396 bp	1.0	67.5