Hotspot

rbcL-psaI

LSC · 58293-59113 · 821 bp

Back to Garnotia

Pi 0.0102

MarkerSeek score 53.80

Primer available yes

Label rbcL-psaI

Feature Metadata

label_name: rbcL-psaI
feature_type: igs
region: LSC
length_bp: 821
variable_sites: 15
pi: 0.0102
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-psaI_p1	AAATTTGACTTCGCTCCGGT	GTCATTGCAATTGCCGGAAA	45.0	45.0	58.1	57.9	928-1019 bp	1.0	79.7
rbcL-psaI_p2	CAAATTTGACTTCGCTCCGG	GTCATTGCAATTGCCGGAAA	50.0	45.0	57.8	57.9	929-1020 bp	1.0	78.6
rbcL-psaI_p3	AAATTTGACTTCGCTCCGGT	GAAGTCATTGCAATTGCCGG	45.0	50.0	58.1	58.4	931-1022 bp	1.0	77.4
rbcL-psaI_p4	AAATTTGACTTCGCTCCGGT	GGAAGTCATTGCAATTGCCG	45.0	50.0	58.1	58.4	932-1023 bp	1.0	77.4
rbcL-psaI_p5	CAAATTTGACTTCGCTCCGG	GAAGTCATTGCAATTGCCGG	50.0	50.0	57.8	58.4	932-1023 bp	1.0	76.3