Hotspot

trnD-GUC-psbM

LSC · 17266-18320 · 1055 bp

Back to Garnotia

Pi 0.00932

MarkerSeek score 59.40

Primer available yes

Label trnD-GUC-psbM

Feature Metadata

label_name: trnD-GUC-psbM
feature_type: igs
region: LSC
length_bp: 1055
variable_sites: 17
pi: 0.00932
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnD-GUC-psbM_p1	AATAGGCATGCCATACACCC	GGCAGTAGGAACTAGAATGAACA	50.0	43.5	57.7	57.9	1213-1231 bp	1.0	54.5
trnD-GUC-psbM_p2	TTCAATTGGTCAGAGCACCG	GGCAGTAGGAACTAGAATGAACA	50.0	43.5	58.5	57.9	1179-1197 bp	1.0	53.0
trnD-GUC-psbM_p3	AATAGGCATGCCATACACCC	AGGCAGTAGGAACTAGAATGAAC	50.0	43.5	57.7	57.7	1214-1232 bp	1.0	52.4
trnD-GUC-psbM_p4	AATAGGCATGCCATACACCC	AGGCAGTAGGAACTAGAATGA	50.0	42.9	57.7	55.5	1214-1232 bp	1.0	51.1
trnD-GUC-psbM_p5	TTCAATTGGTCAGAGCACCG	AGGCAGTAGGAACTAGAATGAAC	50.0	43.5	58.5	57.7	1180-1198 bp	1.0	51.0