Hotspot

trnG-UCC-trnT-GGU

LSC · 14068-16041 · 1974 bp

Back to Garnotia

Pi 0.011
MarkerSeek score 60.90
Primer available yes
Label trnG-UCC-trnT-GGU

Feature Metadata

label_name
trnG-UCC-trnT-GGU
feature_type
igs
region
LSC
length_bp
1974
variable_sites
40
pi
0.011
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.98
markerseek_score
60.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnG-UCC-trnT-GGU_p1 ACGAATCACACTTTTACCACT ACCACTAAGTGAAAAGCCCT 38.1 45.0 55.5 56.7 2075-2096 bp 1.0 44.5
trnG-UCC-trnT-GGU_p2 ACGAATCACACTTTTACCACT TGAACCGATGACTTATGCCT 38.1 45.0 55.5 56.6 2045-2066 bp 1.0 44.4
trnG-UCC-trnT-GGU_p3 ACGAATCACACTTTTACCACT CCGATGACTTATGCCTTACCA 38.1 47.6 55.5 57.5 2041-2062 bp 1.0 43.7
trnG-UCC-trnT-GGU_p4 ACGAATCACACTTTTACCACT ACCGATGACTTATGCCTTACC 38.1 47.6 55.5 57.5 2042-2063 bp 1.0 43.7
trnG-UCC-trnT-GGU_p5 GAACGAATCACACTTTTACCACT ACCACTAAGTGAAAAGCCCT 39.1 45.0 57.3 56.7 2077-2098 bp 1.0 43.6