Hotspot

trnS-GCU-psbD

LSC · 8125-9103 · 979 bp

Back to Garnotia

Pi 0.00681

MarkerSeek score 56.90

Primer available yes

Label trnS-GCU-psbD

Feature Metadata

label_name: trnS-GCU-psbD
feature_type: igs
region: LSC
length_bp: 979
variable_sites: 12
pi: 0.00681
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 56.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-psbD_p1	ATTAGCAATCCGCCGCTTTA	ACAAAAACGAAACGGTCCCT	45.0	45.0	58.0	58.2	1103-1115 bp	1.0	79.2
trnS-GCU-psbD_p2	ATTAGCAATCCGCCGCTTTA	GAAACGGTCCCTTCGTAACC	45.0	55.0	58.0	58.6	1095-1107 bp	1.0	76.6
trnS-GCU-psbD_p3	GCTTTAGTCCACTCAGCCAT	ACAAAAACGAAACGGTCCCT	50.0	45.0	57.6	58.2	1089-1101 bp	1.0	76.2
trnS-GCU-psbD_p4	ATTAGCAATCCGCCGCTTTA	CTTCGTAACCAGTCGTCCAT	45.0	50.0	58.0	57.4	1085-1097 bp	1.0	76.0
trnS-GCU-psbD_p5	AGTCCACTCAGCCATCTCTC	ACAAAAACGAAACGGTCCCT	55.0	45.0	58.5	58.2	1084-1096 bp	1.0	75.4