Hotspot

ycf1-trnN-GUU

IRa · 127773-128875 · 1103 bp

Back to Garidella

Pi 0.011

MarkerSeek score 63.40

Primer available yes

Label ycf1-trnN-GUU

Feature Metadata

label_name: ycf1-trnN-GUU
feature_type: igs
region: IRa
length_bp: 1103
variable_sites: 12
pi: 0.011
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 63.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1-trnN-GUU_p1	TAAAGTCCAACCCCAACCAC	GTAGGATTCGAACCCACGAC	50.0	55.0	57.6	58.1	1252-1259 bp	1.0	78.0
ycf1-trnN-GUU_p2	TAAAGTCCAACCCCAACCAC	AGTAGGATTCGAACCCACGA	50.0	50.0	57.6	58.2	1253-1260 bp	1.0	77.4
ycf1-trnN-GUU_p3	CCCCAACCACCGAATTGATT	GTAGGATTCGAACCCACGAC	50.0	55.0	58.4	58.1	1242-1249 bp	1.0	77.4
ycf1-trnN-GUU_p4	CCCCAACCACCGAATTGATT	AGTAGGATTCGAACCCACGA	50.0	50.0	58.4	58.2	1243-1250 bp	1.0	76.8
ycf1-trnN-GUU_p5	TAAAGTCCAACCCCAACCAC	CCCAAGTAGGATTCGAACCC	50.0	55.0	57.6	57.7	1257-1264 bp	1.0	76.0