Hotspot

ndhG-ndhI

SSC · 117630-118023 · 394 bp

Back to Garidella

Pi 0.0203

MarkerSeek score 66.10

Primer available yes

Label ndhG-ndhI

Feature Metadata

label_name: ndhG-ndhI
feature_type: igs
region: SSC
length_bp: 394
variable_sites: 8
pi: 0.0203
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG-ndhI_p1	CGTGTATTGGCCCAGGTAAA	AGGTCGCTTGCCAATATCAG	50.0	50.0	57.9	58.0	537-547 bp	1.0	79.9
ndhG-ndhI_p2	ACCGCCCACTATAAGTACCA	AGGTCGCTTGCCAATATCAG	50.0	50.0	57.8	58.0	581-591 bp	1.0	79.5
ndhG-ndhI_p3	AAATCGTGTATTGGCCCAGG	AGGTCGCTTGCCAATATCAG	50.0	50.0	58.2	58.0	541-551 bp	1.0	78.9
ndhG-ndhI_p4	CGTGTATTGGCCCAGGTAAA	GGTCGCTTGCCAATATCAGT	50.0	50.0	57.9	58.3	536-546 bp	1.0	77.6
ndhG-ndhI_p5	ACCGCCCACTATAAGTACCA	GGTCGCTTGCCAATATCAGT	50.0	50.0	57.8	58.3	580-590 bp	1.0	77.1