Hotspot

ycf2-trnL-CAA

IRb · 93669-94686 · 1018 bp

Back to Garidella

Pi 0.0128

MarkerSeek score 61.50

Primer available yes

Label ycf2-trnL-CAA

Feature Metadata

label_name: ycf2-trnL-CAA
feature_type: igs
region: IRb
length_bp: 1018
variable_sites: 13
pi: 0.0128
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf2-trnL-CAA_p1	AAAGAGATGGCTTTTCCCGG	AAATGGTAGACACGCGAGAC	50.0	50.0	58.2	58.0	1160 bp	1.0	79.5
ycf2-trnL-CAA_p2	AAAGAGATGGCTTTTCCCGG	TGAAATGGTAGACACGCGAG	50.0	50.0	58.2	58.0	1162 bp	1.0	79.5
ycf2-trnL-CAA_p3	ATGGCTTTTCCCGGATGAAA	AAATGGTAGACACGCGAGAC	45.0	50.0	57.8	58.0	1154 bp	1.0	79.0
ycf2-trnL-CAA_p4	TGGCTTTTCCCGGATGAAAT	AAATGGTAGACACGCGAGAC	45.0	50.0	57.8	58.0	1153 bp	1.0	79.0
ycf2-trnL-CAA_p5	TGGCTTTTCCCGGATGAAAT	TGAAATGGTAGACACGCGAG	45.0	50.0	57.8	58.0	1155 bp	1.0	79.0