Hotspot

rpl14-rpl16

LSC · 81751-81869 · 119 bp

Back to Garidella

Pi 0.0168

MarkerSeek score 62.20

Primer available yes

Label rpl14-rpl16

Feature Metadata

label_name: rpl14-rpl16
feature_type: igs
region: LSC
length_bp: 119
variable_sites: 2
pi: 0.0168
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl14-rpl16_p1	AACGTCACCAATACGAGCAT	AGCTGCGTCAAAAATGCCTA	45.0	45.0	57.6	58.5	282 bp	1.0	73.8
rpl14-rpl16_p2	AACGTCACCAATACGAGCAT	TAGCTGCGTCAAAAATGCCT	45.0	45.0	57.6	58.5	283 bp	1.0	73.8
rpl14-rpl16_p3	CATCAATTCTCTAGCCCCGC	AGCTGCGTCAAAAATGCCTA	55.0	45.0	58.5	58.5	228 bp	1.0	73.1
rpl14-rpl16_p4	AACGTCACCAATACGAGCAT	ATAGCTGCGTCAAAAATGCC	45.0	45.0	57.6	57.4	284 bp	1.0	73.0
rpl14-rpl16_p5	AACGTCACCAATACGAGCAT	GCTGCGTCAAAAATGCCTAT	45.0	45.0	57.6	57.4	281 bp	1.0	73.0