Hotspot

psbE-petL

LSC · 64880-66120 · 1241 bp

Back to Garidella

Pi 0.00968

MarkerSeek score 64.40

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1241
variable_sites: 12
pi: 0.00968
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	ACGAATAACCAACCCGCAAT	GTCGTATCTTGCTCAGACCA	45.0	50.0	58.2	57.1	1442-1457 bp	1.0	71.8
psbE-petL_p2	CGAATAACCAACCCGCAATG	GTCGTATCTTGCTCAGACCA	50.0	50.0	57.8	57.1	1441-1456 bp	1.0	71.7
psbE-petL_p3	AACGAATAACCAACCCGCAA	GTCGTATCTTGCTCAGACCA	45.0	50.0	58.4	57.1	1443-1458 bp	1.0	70.0
psbE-petL_p4	ACGAATAACCAACCCGCAAT	GTCGTATCTTGCTCAGACCAA	45.0	47.6	58.2	57.8	1442-1457 bp	1.0	69.5
psbE-petL_p5	ACGAATAACCAACCCGCAAT	AGTCGTATCTTGCTCAGACC	45.0	50.0	58.2	56.8	1443-1458 bp	1.0	69.4