Hotspot

trnM-CAU-atpE

LSC · 52924-53030 · 107 bp

Back to Garidella

Pi 0.0187

MarkerSeek score 63.20

Primer available yes

Label trnM-CAU-atpE

Feature Metadata

label_name: trnM-CAU-atpE
feature_type: igs
region: LSC
length_bp: 107
variable_sites: 2
pi: 0.0187
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnM-CAU-atpE_p1	GGCAGGAGTCATTGGTTCAA	AGGACGCGAATAGAGGCTAT	50.0	50.0	58.1	58.1	178 bp	1.0	79.5
trnM-CAU-atpE_p2	GGCAGGAGTCATTGGTTCAA	CTCTGAAACGAGCTAGGACG	50.0	55.0	58.1	57.8	192 bp	1.0	78.7
trnM-CAU-atpE_p3	GGCAGGAGTCATTGGTTCAA	GGACGCGAATAGAGGCTATC	50.0	55.0	58.1	57.7	177 bp	1.0	78.1
trnM-CAU-atpE_p4	TTGCTTTCATACGGCAGGAG	AGGACGCGAATAGAGGCTAT	50.0	50.0	58.3	58.1	190 bp	1.0	78.0
trnM-CAU-atpE_p5	TTGCTTTCATACGGCAGGAG	CTCTGAAACGAGCTAGGACG	50.0	55.0	58.3	57.8	204 bp	1.0	77.2