Hotspot

trnF-GAA-ndhJ

LSC · 48451-49187 · 737 bp

Back to Garidella

Pi 0.00956

MarkerSeek score 64.90

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 737
variable_sites: 7
pi: 0.00956
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 64.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	CCTCGTGTCACCAGTTCAAA	GATAATCATCCGCGCCTGAA	50.0	50.0	58.1	58.2	877-882 bp	1.0	79.0
trnF-GAA-ndhJ_p2	CCTCGTGTCACCAGTTCAAA	GAAAGTTGGATAGGGTGGCC	50.0	55.0	58.1	58.2	841-846 bp	1.0	78.8
trnF-GAA-ndhJ_p3	ATCGGGAAGAGTCGGGATAG	GATAATCATCCGCGCCTGAA	55.0	50.0	58.1	58.2	926-931 bp	1.0	78.7
trnF-GAA-ndhJ_p4	CCTCGTGTCACCAGTTCAAA	ATAATCATCCGCGCCTGAAA	50.0	45.0	58.1	57.7	876-881 bp	1.0	78.6
trnF-GAA-ndhJ_p5	CCTCGTGTCACCAGTTCAAA	ATGATAATCATCCGCGCCTG	50.0	50.0	58.1	58.3	879-884 bp	1.0	78.5