Hotspot

atpH-atpI

LSC · 12446-13279 · 834 bp

Back to Garidella

Pi 0.00607

MarkerSeek score 63.40

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 834
variable_sites: 5
pi: 0.00607
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 63.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	TCCAGGTCCAATAGAAGCCA	ATTTTCGCAACTTTAGCCGC	50.0	45.0	58.0	58.0	957-966 bp	1.0	80.8
atpH-atpI_p2	AACGGAAGCAGCAGAAATCA	ATTTTCGCAACTTTAGCCGC	45.0	45.0	58.1	58.0	912-921 bp	1.0	80.3
atpH-atpI_p3	TACCTTGACCAACTCCAGGT	ATTTTCGCAACTTTAGCCGC	50.0	45.0	57.9	58.0	970-979 bp	1.0	80.2
atpH-atpI_p4	TCCAGGTCCAATAGAAGCCA	GGGGAATCTATGGAGGGTCA	50.0	55.0	58.0	57.9	927-936 bp	1.0	80.1
atpH-atpI_p5	GATACCTTCTACGGCTTGGC	ATTTTCGCAACTTTAGCCGC	55.0	45.0	58.1	58.0	999-1008 bp	1.0	80.0