Hotspot

ndhB

Genome · 97071-99288 · 2218 bp

Back to Gardneria

Pi 0.00316

MarkerSeek score 59.10

Primer available yes

Label ndhB

Feature Metadata

label_name: ndhB
feature_type: gene
region: Genome
length_bp: 2218
variable_sites: 7
pi: 0.00316
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhB_p1	ATAACCCTAGCCCATTCCCA	TCCTGGAAAGTTCGACACAA	50.0	45.0	57.8	57.0	2375-2376 bp	1.0	70.6
ndhB_p2	ATAACCCTAGCCCATTCCCA	TTCCTGGAAAGTTCGACACA	50.0	45.0	57.8	57.0	2376-2377 bp	1.0	70.6
ndhB_p3	TAACCCTAGCCCATTCCCAT	TCCTGGAAAGTTCGACACAA	50.0	45.0	57.8	57.0	2374-2375 bp	1.0	70.6
ndhB_p4	TAACCCTAGCCCATTCCCAT	TTCCTGGAAAGTTCGACACA	50.0	45.0	57.8	57.0	2375-2376 bp	1.0	70.6
ndhB_p5	CCCTAGCCCATTCCCATTTT	TCCTGGAAAGTTCGACACAA	50.0	45.0	57.5	57.0	2371-2372 bp	1.0	68.5