Hotspot

ndhF-rpl32

Genome · 114528-115311 · 784 bp

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Pi 0.0128

MarkerSeek score 60.70

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: Genome
length_bp: 784
variable_sites: 10
pi: 0.0128
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	GTTGCCGTCGGAAAAAGAAG	AAGGCTTTTAACGCTGTCCA	50.0	45.0	58.0	58.0	977-980 bp	1.0	80.4
ndhF-rpl32_p2	GTTGCCGTCGGAAAAAGAAG	AGGCTTTTAACGCTGTCCAA	50.0	45.0	58.0	58.0	976-979 bp	1.0	80.4
ndhF-rpl32_p3	TGCCGTCGGAAAAAGAAGAA	AAGGCTTTTAACGCTGTCCA	45.0	45.0	58.0	58.0	975-978 bp	1.0	80.3
ndhF-rpl32_p4	TGCCGTCGGAAAAAGAAGAA	AGGCTTTTAACGCTGTCCAA	45.0	45.0	58.0	58.0	974-977 bp	1.0	80.3
ndhF-rpl32_p5	TTGCCGTCGGAAAAAGAAGA	AGGCTTTTAACGCTGTCCAA	45.0	45.0	58.0	58.0	975-978 bp	1.0	80.3