Hotspot

ndhC-trnV-UAC

Genome · 52650-53867 · 1218 bp

Back to Gardneria

Pi 0.00911

MarkerSeek score 55.60

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: Genome
length_bp: 1218
variable_sites: 11
pi: 0.00911
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 55.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	TCATATTCGTAAAGCAGAAACA	TAGCGAGAAGGTCTACGGTT	31.8	50.0	53.9	57.9	1270-1279 bp	1.0	41.4
ndhC-trnV-UAC_p2	TCATATTCGTAAAGCAGAAACA	TTAGCGAGAAGGTCTACGGT	31.8	50.0	53.9	57.9	1271-1280 bp	1.0	41.4
ndhC-trnV-UAC_p3	AGCCAAAATAGGAATAAGACTTGA	TAGCGAGAAGGTCTACGGTT	33.3	50.0	56.1	57.9	1319-1328 bp	1.0	41.3
ndhC-trnV-UAC_p4	AGCCAAAATAGGAATAAGACTTGA	TTAGCGAGAAGGTCTACGGT	33.3	50.0	56.1	57.9	1320-1329 bp	1.0	41.3
ndhC-trnV-UAC_p5	AGCCAAAATAGGAATAAGACTTGA	AGAAGGTCTACGGTTCGAGT	33.3	50.0	56.1	57.8	1314-1323 bp	1.0	41.3