Hotspot

trnT-UGU-trnL-UAA

Genome · 48453-49220 · 768 bp

Back to Gardneria

Pi 0.00134

MarkerSeek score 62.30

Primer available yes

Label trnT-UGU-trnL-UAA

Feature Metadata

label_name: trnT-UGU-trnL-UAA
feature_type: igs
region: Genome
length_bp: 768
variable_sites: 1
pi: 0.00134
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 62.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-UGU-trnL-UAA_p1	GCGATGCTCTAACCTCTGAG	GCGTCTACCTATTTCGCCAT	55.0	50.0	57.9	57.8	802-824 bp	1.0	80.0
trnT-UGU-trnL-UAA_p2	TAGGAAAAAGCCGGCTATCG	GCGTCTACCTATTTCGCCAT	50.0	50.0	57.8	57.8	853-875 bp	1.0	79.1
trnT-UGU-trnL-UAA_p3	GGAATCGAACCGATGACCAT	GCGTCTACCTATTTCGCCAT	50.0	50.0	57.8	57.8	834-856 bp	1.0	79.1
trnT-UGU-trnL-UAA_p4	GCGATGCTCTAACCTCTGAG	GGTTTCCATACCAAGGCTCA	55.0	50.0	57.9	57.5	839-861 bp	1.0	77.0
trnT-UGU-trnL-UAA_p5	ATGCGATGCTCTAACCTCTG	GCGTCTACCTATTTCGCCAT	50.0	50.0	57.5	57.8	804-826 bp	1.0	76.9