Hotspot

rps2-rpoC2

Genome · 17324-17573 · 250 bp

Back to Gardneria

Pi 0.0127

MarkerSeek score 64.50

Primer available yes

Label rps2-rpoC2

Feature Metadata

label_name: rps2-rpoC2
feature_type: igs
region: Genome
length_bp: 250
variable_sites: 3
pi: 0.0127
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 64.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps2-rpoC2_p1	CCTGCCTCCATCATCTCTTC	TTCGAGGGGGAAATGAGAGA	55.0	50.0	57.5	57.8	404-417 bp	1.0	76.8
rps2-rpoC2_p2	ATGGCCAAAATGAACCCCTG	TTCGAGGGGGAAATGAGAGA	50.0	50.0	58.7	57.8	420-433 bp	1.0	75.2
rps2-rpoC2_p3	CATGGCCAAAATGAACCCCT	TTCGAGGGGGAAATGAGAGA	50.0	50.0	58.7	57.8	421-434 bp	1.0	75.2
rps2-rpoC2_p4	CCTGCCTCCATCATCTCTTC	TCGAGGGGGAAATGAGAGAT	55.0	50.0	57.5	57.5	403-416 bp	1.0	74.9
rps2-rpoC2_p5	CCCTGCCTCCATCATCTCTT	TTCGAGGGGGAAATGAGAGA	55.0	50.0	58.9	57.8	405-418 bp	1.0	74.2